The distribution of radioactivity in a female monkey was firm after intravenous injection of 300 mg/kg 35S-sodium thioglycolate.
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). The absence of pink in the remainder of the tube indicates the absence of oxygen and an acceptable environment for strict anaerobes. The growth with the organisms in different regions in the check tubes will count on the oxygen requirement qualities of People organisms:
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reason: the usage of thioglycollate broth permits expansion of anaerobic microorganisms. Growth patterns may also help distinguish aerotolerance and oxygen requirements of microorganisms. Media: has glucose, cysteine, and sodium thioglycollate (oxygen minimizing agent) Reagents/Indicators: Resazurin Mechanism/Reactions: This is a nutritive medium using a cutting down agent (sodium thioglycollate), which, because of a chemical reaction, eliminates oxygen from the broth.
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Sodium thioglycollate work as a lessening agent and neutralizes the harmful consequences of mercurial preservatives and peroxides formed within the medium, thereby selling anaerobiosis, and producing the medium suitable to check materials that contains heavy metals.